Clostridioides difficile: Relevance, Ribotypes, and Endoscope Reprocessing
Clostridioides difficile is one of the most common nosocomial pathogens and is also present outpatient settings.
The hypervirulent ribotype 027 results in serious disease outcomes and increased mortality in numerous countries worldwide.
If flexible endoscopes are cleaned and disinfected thoroughly after each patient use, based on current evidence, endoscopic procedures pose no risk in terms of the transmission of Clostridioides difficile.
Clostridioides (formerly Clostridium) difficile (C. difficile) is considered one of the most common pathogens of nosocomial and antibiotic-associated diarrhea. The number of C. difficile infections (CDIs) occurring globally has increased substantially since 2000. For example, according to one study from 2014, the pathogen caused 12.1% of all reported nosocomial infections in US hospitals 9. CDIs prolong hospitalization in the United States by 2.8 to 10.4 days and cause costs in the amount of 42,000 US dollars per case. Elderly patients and individuals with serious underlying illnesses and comorbidities are at particular risk. However, C. difficile is also a growing problem outpatient settings. Thus, an increased incidence of CDIs has also been observed in outpatient settings for some years.
Spread of hypervirulent ribotype 027
At the same time, an increased number of severe cases and outbreaks has been observed. This development can be attributed in particular to the spread of a hypervirulent strain of the C. difficile pathogen, ribotype 027 (RT 027). Originating in North America, RT 027 has spread along two distinct epidemic lines to regions that include Europe, Australia, and South Korea, as has been shown in genetic analyses. RT 027 exhibits an increased production of the toxins that trigger disease. For example, this strain releases 16 times more enterotoxin and 23 times more cytotoxin B. RT 027 also produces the binary toxin CDT. Infections with the strain result in a substantially higher mortality rate. RT 027 is, furthermore, resistant to several antibiotics, especially the fluoroquinolones.
Ribotypes 001, 014, 017, 078, and 176 are other C. difficile strains for which severe cases and complications have also been described. The pathogenic strains also include ribotype 106. First identified in the UK, the strain has now spread worldwide and superseded RT027 as the most prevalent strain in the USA. Studies have concluded that RT 106 causes less severe illness than RT 027 1. However, patients infected with the RT 106 strain are significantly more likely to suffer multiple CDI relapses.
C. difficile in endoscopy
Despite the high contagiousness of C. difficile, there has to date been only one documented cases of possible transmission after a colonoscopy 8. The risk of developing C. difficile-associated diarrhea (CDAD) after a gastrointestinal endoscopy procedure is therefore considered to be low. However, another study showed that 10 out of 15 colonoscopes were contaminated with C. difficile immediately after being used for CDAD patients 7.
In terms of patient safety, thorough manual cleaning followed by automated washing and disinfection in endoscope washer-disinfectors (EWD/AER) play a key role. Validated automated processes are used to safely reprocess endoscopes, and the processes used for EWD/AER must be proven to be effective against bacterial spores in accordance with ISO 15883-4. In addition, with EN 17126, a new testing method has been available since 2018 for demonstrating the sporicidal efficacy of chemical disinfectants, including spores from C. difficile ribotype 027 used as test microorganism. According to this standard, the disinfectant must reduce the number of spores by at least 4 log 10 within 60 minutes.
Carlson TJ et al. Clostridioides difficile ribotype 106: a systematic review of the antimicrobial susceptibility, genetics, and clinical outcomes of this common worldwide strain, Anaerobe. 2020 April; 62: 102142.
De Roo AC et al. Clostridium difficile Infection: An Epidemiology Update, Clin Colon Rectal Surg 2020;33:49–57.
EN 15883:2019-06, Washer-disinfectors - Part 4: Requirements and tests for washer-disinfectors employing chemical disinfection for thermolabile endoscopes (ISO 15883-4:2018), Brussels: CEN - Comité Européen de Normalisation, 2019.
EN 17126:2018, Chemical disinfectants and antiseptics Quantitative suspension test for the evaluation of sporicidal activity of chemical disinfectants in the medical area — Test method and requirements (phase 2, step 1), Brussels: CEN - Comité Européen de Normalisation, 2018.
He M et al. Emergence and global spread of epidemic healthcare-associated Clostridium difficile, Nat Genet. 2013 Jan; 45(1): 109–113
Hygienemaßnahmen bei Clostridioides difficile-Infektion (CDI), Empfehlung der Kommission für Krankenhaushygiene und Infektionsprävention (KRINKO) beim Robert Koch-Institut, Bundesgesundheitsbl 2019 · 62:906–923.
Kampf G Clostridium difficile – what should be considered for an effective disinfection? Hyg Med 2008; 33 : 153–159
Kovaleva J et al. Transmission of Infection by Flexible Gastrointestinal Endoscopy and Bronchoscopy, Clin Microbiol Rev. 2013 Apr;26(2):231-54.
Magill SS et al. Multistate point-prevalence survey of health care-associated infections, N Engl J Med 2014; 370:1198-1208.
McDonald LC et al. Clinical Practice Guidelines for Clostridium difficile Infection in Adults and Children: 2017 Update by the Infectious Diseases Society of America (IDSA) and Society for Healthcare Epidemiology of America (SHEA), Clinical Practice Guidelines for Clostridium difficile Infection ∙ CID 2018:66.
Pépin J et al. Mortality attributable to nosocomial Clostridium difficile–associated disease during an epidemic caused by a hypervirulent strain in Quebec, CMAJ. 2005 Oct 25; 173(9): 1037–1042